Journal: PLoS ONE
Article Title: Development of a Quantitative Bead Capture Assay for Soluble IL-7 Receptor Alpha in Human Plasma
doi: 10.1371/journal.pone.0006690
Figure Lengend Snippet: Recombinant sCD127-Fc chimera was serially diluted from 0.1 to 1000 ng/mL in assay diluent and used as the assay's calibrator. The recombinant sCD127 was captured on beads conjugated with sCD127 polyconal antibodies. Bound sCD127 was detected with a biotinylated monoclonal anti-CD127 and streptavidin-PE. The curve was resolved using a 5-parameter logistic equation (MasterPlex QT software). Insert: Standard curve inter-assay variation from 12 assays done in duplicates. The curve's range was 3.2–1000 ng/mL.
Article Snippet: The bead analysis was done using the MiraiBio software (MasterPlex CT, version 1) and the standard curves were generated using a 5-parameter logistic curve fitting equation (MasterPlex QT software, version 4, MiraiBio).
Techniques: Recombinant, Software, Inter Assay